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TriLink BioTechnologies——高品質常規/特殊核苷酸產品
TriLink BioTechnologies公司是世界的核酸修飾技術領域的，成立于1996年，總部設在San Diego，California。TriLink公司致力于高品質核酸修飾產品的研發和生產，提供包括核苷酸、常規及核苷三磷酸特殊修飾的寡核苷酸定制（oligonucleotides），m RNA合成，CleanAmp?PCR產品，phosphoramidites和其他小分子在內的眾多產品。近二十年來，TriLink一直是診斷和OEM市場核酸產品供應的行業，產品應用于基因治療，核苷類化療，寡核苷酸治療和診斷領域。此外，TriLink還可提供特殊核苷酸、m RNA定制，合同研究服務和ISO/ QSR標準的cGMP生產設施。TriLink完善的產品及研發服務解決方案有助于推動藥物發現和生物醫學研究。

經過18年的發展， TriLink已經成為高品質RNA合成領域的，為廣大科研工作者提供的mRNA及長鏈RNA（長可達幾個Kb），并且定制修飾。同時我們也提供成品mRNA產品，包括報告基因及 mRNA表達因子。

Reporter Gene mRNA

Reporter genes are commonplace tools in cell biology research. TriLink has developed optimized capped (Cap 0 and Cap 1) and polyadenylated mRNA expressing reporters. These reporter mRNAs can be used as controls to study mRNA transfection and expression in mammalian cells using a variety of assays including fluorescence microscopy, quantitative fluorometry and bioluminescent imaging, as well as fluorescence-activated cell sorting (FACS).

產品如下：

L-7601
CleanCap™ EGFP mRNA
CleanCap™ Enhanced Green Fluorescent Protein mRNA 

mRNA Length: 996 nucleotides

Concentration: 1.0 mg/mL

Buffer: 1 mM Sodium Citrate, pH 6.4

Identity & Purity:

Agarose Gel Mobility; Pass

Concentration ± 5%; Pass

Product InsertSDS

The EGFP mRNA will express an enhanced version of the green fluorescent protein, originally isolated from the jellyfish, Aequorea victoria. EGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.

This mRNA is capped using CleanCap™, TriLink's proprietary co-transciptional capping method, which results in the naturally occuring Cap 1 structure with high capping efficiency. It is polyadenylated and optimized for mammalian systems. It mimics a fully processed mature mRNA.

Sold for research use only under license from Life Technologies.

Certificate(s) of Analysis

TD-OF01A

Not for resale without express written permission. Not for use in humans. No license under any patent or patent pending is granted or implied by the purchase of any TriLink product. TriLink does not warrant that the use or sale of the products delivered hereunder will not infringe the claims of any United States or other patents or patents pending covering the use of the product alone or in combination with other products or in the operation of any process. All and any use of TriLink product is the purchaser’s sole responsibility.

L-7201
CleanCap™ EGFP mRNA (5moU)
CleanCap™ Enhanced Green Fluorescent Protein mRNA (5-methoxyuridine) 

mRNA Length: 996 nucleotides

Concentration: 1.0 mg/mL

Buffer: 1 mM Sodium Citrate, pH 6.4

Identity & Purity:

Agarose Gel Mobility; Pass

Concentration ± 5%; Pass

Product Inser

SDS         

The EGFP mRNA will express an enhanced version of the green fluorescent protein, originally isolated from the jellyfish, Aequorea victoria. EGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.

This mRNA is capped using CleanCap™, TriLink's proprietary co-transciptional capping method, which results in the naturally occuring Cap 1 structure with high capping efficiency. It is polyadenylated, modified with 5-methoxyuridine and optimized for mammalian systems. It mimics a fully processed mature mRNA.

Certificate(s) of Analysis

TD-OA01A-1

TD-OA01A

T1-CHI01A-1

L-7201 is a replacement product for L-6101 and L-6126.

Sold for research use only under license from Life Technologies.

Not for resale without express written permission. Not for use in humans. No license under any patent or patent pending is granted or implied by the purchase of any TriLink product. TriLink does not warrant that the use or sale of the products delivered hereunder will not infringe the claims of any United States or other patents or patents pending covering the use of the product alone or in combination with other products or in the operation of any process. All and any use of TriLink product is the purchaser’s sole responsibility.

L-7701
CleanCap™ Cyanine 5 EGFP mRNA (5moU)
CleanCap™ Cyanine 5 Enhanced Green Fluorescent Protein mRNA (5-methoxyuridine) 

mRNA Length: 996 nucleotides

Concentration: 1.0 mg/mL

Buffer: 1 mM Sodium Citrate, pH 6.4

Identity & Purity:

Agarose Gel Mobility; Pass

Concentration ± 5%; Pass

Product Insert

SDS

Cyanine 5 EGFP mRNA is designed for the analysis of mRNA delivery and translation efficiency. The EGFP mRNA will express an enhanced version of the green fluorescent protein, originally isolated from the jellyfish, Aequorea victoria. EGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm. When labeled with cyanine 5, EGFP mRNA can be directly visualized. Cyanine 5 EGFP mRNA is an ideal molecule to determine mRNA delivery and localization independent of translation.

Cyanine 5 is a synthetic fluorescent dye with maximum excitation and emission wavelengths of 650 nm and 670 nm respectively. TriLink’s CleanCap Cyanine 5 EGFP mRNA is transcribed with Cyanine 5-UTP:5-Methoxy-UTP at a ratio of 1:3. Substitution in this ratio results in mRNA that is easily visualized and can still be translated in cell culture. Translation efficiency correlates inversely with Cyanine 5-UTP substitution.

This mRNA is capped using CleanCap™, TriLink's proprietary co-transciptional capping method, which results in the naturally occuring Cap 1 structure with high capping efficiency. It is polyadenylated, modified with 5-methoxyuridine and optimized for mammalian systems. It mimics a fully processed mature mRNA.

Certificate(s) of Analysis

TD-OF03A

L-7701 is a replacement for L-6402. 

Sold for research use only under license from Life Technologies.

Not for resale without express written permission. Not for use in humans. No license under any patent or patent pending is granted or implied by the purchase of any TriLink product. TriLink does not warrant that the use or sale of the products delivered hereunder will not infringe the claims of any United States or other patents or patents pending covering the use of the product alone or in combination with other products or in the operation of any process. All and any use of TriLink product is the purchaser’s sole responsibility.