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Dianova DIA-900-BIOT說明書
Art.-Nr.
DIA-900-BIOT
特異性
他的Epitop-Tag
物種反應性
全部
重組
寄主物種
老鼠
同型
的IgG1
克隆
13/45/31
克隆性（單克隆/多克隆）
單克隆
應用
免疫組化（冷凍切片）
免疫熒光
流式細胞儀
Western Blot
免疫組織化學（IHC）
共軛
生物素
產品系列/主題
表位 - 標簽，Haptens和標簽
有可能的使用
僅供研究使用
溫度 - 儲存
2-8℃
搜索代碼
DIA935
制造商/品牌
dianova
反應
小鼠單克隆抗（His）6-標簽抗體，克隆13/45 / 31-2（H.Zentgraf / DKFZ Heidelberg，Germany），特異性地檢測細胞和復雜細胞裂解物中的任何類型的組氨酸標記的蛋白質。具有3x 10-10M（Biacore TM分析）的值，抗體的親和力是顯著的。該單克隆抗體與含有至少6個組氨酸殘基的表位的重組蛋白特異性反應，所述表位位于N末端，C末端或位于兩個終點。更高數量的組氨酸殘基導致抗體的結合親和力增加，例如具有10個組氨酸殘基的表達產物使親和力增加約10至20倍。在大多數情況下，抗體結合不需要額外的側翼氨基酸，
背景
重組蛋白質用于分子生物學中的各種目的，例如用于研究蛋白質 - 核酸或蛋白質 - 蛋白質相互作用機制的抗體的產生。已經建立了許多原核表達載體，使得能夠合成目的蛋白質作為與肽的融合，從而促進純化。重組蛋白在大腸桿菌中作為與鄰近組氨酸殘基的融合蛋白的表達是流行的方法之一，因為蛋白質具有有用的屬性。組氨酸 - 標簽基序通過螯合作用對Ni2 +的親和力強且足夠選擇性，使得能夠通過在Ni2 + -NTA吸附劑上的親和色譜法將蛋白質純化至均一。
檢測（HIS）6-p53 Hela細胞裂解物（根據標準程序用HIS標記的蛋白質載體轉染，SDS-PAGE和蛋白質印跡）。靈敏度：在1：100抗體稀釋度下對8μg總蛋白質中的1ng蛋白質進行特異性檢測/對于較不敏感的檢測，抗體分析高達1：10.000（約6ng HIS標記的蛋白質）。
參考文獻：
克隆13/45/31是世界上引用多的His-Tag抗體之一。
發表：
1.Zentgraf H，Frey M，Schwinn S，Tessmer C，Willemann B，Samstag Y，Velhagen I.通過使用高特異性小鼠單克隆抗組氨酸標簽抗體檢測組氨酸標記的融合蛋白。Nucleic Acids Res.16,3347-8,1995。
Dianova2019年產品價格表
貨號 | 品名 | 規格 | 價格 | 品牌 |
DIA-100 | Anti-Fibroblasten (CD90) (Hu) from Mouse (Clone: AS02) | 200µg | 4680 | dianova |
DIA-100-M | Anti-Fibroblasten (CD90) (Hu) from Mouse (Clone: AS02) | 20µg | 0 | dianova |
DIA-120 | Anti-Fibroblasten (CD90) (Hu) from Mouse (Clone: AS02) -FITC | 100test | 5360 | dianova |
DIA-530-P05 | Anti-p53 (Hu) from Mouse (Clone: CC53) | 0.5ml | 4780 | dianova |
DIA-530-M | Anti-p53 (Hu) from Mouse (Clone: CC53) | 0.1ml | 0 | dianova |
DIA-670-P05 | Anti-Ki-67/MIB1 (Hu) from Mouse (Clone: Ki-67P) CE-IVD | 0.5ml | 4780 | dianova |
DIA-670-P1 | Anti-Ki-67/MIB1 (Hu) from Mouse (Clone: Ki-67P) CE-IVD | 1ml | 6500 | dianova |
DIA-670-M | Anti-Ki-67/MIB1 (Hu) from Mouse (Clone: Ki-67P) CE-IVD | 0.1ml | 0 | dianova |
DIA-700-P05 | Anti-GFAP (Hu) from Mouse (Clone: IF3) CE-IVD | 0.5ml | 4780 | dianova |
DIA-700-M | Anti-GFAP (Hu) from Mouse (Clone: IF3) CE-IVD | 0.5ml | 0 | dianova |
DIA-AX1 | Anti-ATRX (Hu) from Mouse (Clone: AX1) | 0.5ml | 6760 | dianova |
DIA-CAL-100 | Anti-CALRETICULIN mutation (Hu) from Mouse (Clone CAL2) | 0.1ml | 5960 | dianova |
DIA-CAL-250 | Anti-CALRETICULIN mutation (Hu) from Mouse (Clone CAL2) | 0.25ml | 13960 | dianova |
DIA-H09 | Anti-IDH1 R132H (Hu) from Mouse (Clone: H09) CE-IVD | 0.5ml | 9640 | dianova |
DIA-H09-M | Anti-IDH1 R132H (Hu) from Mouse (Clone: H09) CE-IVD | 0.1ml | 2840 | dianova |
DIA-H09-L | Anti-IDH1 R132H (Hu) from Mouse (Clone: H09), prediluted, ready-to-use | 8ml | 9640 | dianova |
DIA-W09 | Anti-IDH1 (Hu) from Rat (Clone: W09) | 0.5ml | 4960 | dianova |
DIA-TG1 | Anti-TIGIT (Hu) from Mouse (Clone TG1) | 0.5ml | 20400 | dianova |
DIA-TG1-M | Anti-TIGIT (Hu) from Mouse (Clone TG1) | 0.1ml | 5720 | dianova |
DIA-TC8 | Anti-CD8 (Hu) from Mouse (Clone TC8 ) | 0.5ml | 9200 | dianova |
DIA-P16-OD | Anti-P16 (Hu) from Mouse (Clone JAP16) | 0.1ml | 7360 | dianova |
DIA-303 | Anti-CD3 (Ms, FFPE) from Rat (Clone: HH3E) | 0.5ml | 6900 | dianova |
DIA-310 | Anti-CD31 (Ms, FFPE) from Rat (Clone: SZ31) | 0.5ml | 8040 | dianova |
DIA-404 | Anti-CD4 (Ms, FFPE) from Rat (Clone GHH8) | 0.2ml | 6900 | dianova |
DIA-808 | Anti-CD8a (Ms, FFPE) from Rat (Clone GHH8) | 0.2ml | 6900 | dianova |
DIA-60 | Anti-FasL, soluble (Hu) from Mouse (Clone: 94) | 1ml | 4760 | dianova |
DIA-900-100 | Anti-HIS-Tag (affinity 3x 10‐10M (Biacore™) from Mouse (Clone: 13/45/31) | 100µg | 2960 | dianova |
DIA-900-200 | Anti-HIS Epitope-Tag (affinity 3x 10‐10M (Biacore™) from Mouse (Clone: 13/45/31) | 200µg | 5160 | dianova |
DIA-900-FITC | Anti-HIS Epitope-Tag (affinity 3x 10‐10M (Biacore™) from Mouse (Clone:13/45/31) -FITC | 1ml | 5960 | dianova |
DIA-900-BIOT | Anti-HIS Epitope-Tag (affinity 3x 10‐10M (Biacore™) from Mouse (Clone:13/45/31) -Biotin | 100µg | 3960 | dianova |
DIA-910-200-ABF | Anti-HIS Epitope-Tag (affinity 3x 10‐10M (Biacore™) from Mouse (Clone:13/45/31) Acid/BSA-free | 200µg | 4960 | dianova |
DIA.910-1MG-ABF | Anti-HIS Epitope-Tag (affinity 3x 10‐10M (Biacore™) from Mouse (Clone:13/45/31) Acid/BSA-free | 1mg | 19840 | dianova |
SCR-028841 | IS Adhesion Slides | 50pcs. | 1620 | dianova |
SCR-028855 | IS Adhesion Slides | 5pcs. | 詢價 | dianova |
SCR-028842 | IS Adhesion Slides | 100pcs. | 2440 | dianova |
SCR-038447 | Immunoselect Antifading Mounting Medium | 15ml | 960 | dianova |
SCR-038587 | Immunoselect Antifading Mounting Medium | 1ml | 詢價 | dianova |
SCR-038448 | Immunoselect Antifading Mounting Medium DAPI | 15ml | 1180 | dianova |
SCR-038588 | Immunoselect Antifading Mounting Medium DAPI | 1ml | 詢價 | dianova |
SCR-038449 | Immunoselect Antifading Mounting Medium PI | 15ml | 1180 | dianova |
SCR-072967 | Immunoselect Mounting Medium without hardener | 15ml | 960 | dianova |
SCR-093035 | Immunoselect Antifading Mounting Medium DAPI without hardener | 15ml | 1180 | dianova |
SCR-093036 | Immunoselect Antifading Mounting Medium PI without hardener | 15ml | 1180 | dianova |
DIA-0302-I | p53-auto antibody ELISA-Kit (Human) CE-IVD | 96test | 10960 | dianova |
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